The main topics of the Diamond Sutra are “what is attaching to forms” and “how to leave forms”. In order to reveal its true spirit, this study will investigate problems of attaching to forms and leaving forms in terms of “attaching-mind”, “non-attaching-mind” and “a pure mind arising from non-attaching-mind”. “Attaching-mind” stands for that one’s mind attaches to forms of the outer world. “Non-attaching-mind” appears that when one’s mind won’t attach to forms of the outer world, one therefore will be able to turn his/her attention from forms of the outer world back to his/her inner-self and then penetrating into emptiness will become possible. And, after one attains emptiness, a pure mind will simultaneously appear. The study explores main topics of the Diamond Sutra not only in the light of Buddhist teachings but also physiology to reveal its significance to the public.