Twenty-two years have passed since Venerable Sheng Yen proposed the Fivefold Spiritual Renaissance Campaign (the FSRC, including the Four Fields for Cultivating Peace, the Four Steps for Handling a Problem, the Four Guidelines for Dealing with Desires, the Four Practices for Helping Oneself and Others, and the Four Ways to Cultivate Blessings) as the method to implement Spiritual Environmental Protection (SEP). However, there is no empirical research on the impact of the FSRC on human behavior. In order to investigate the influence of the FSRC, regular practice, and income on the altruistic tendency of economic behavior, we conducted an online survey using snowball sampling and obtained 2,037 valid questionnaires during 2019/5/8~2019/5/13. The main results are as follows: (1) The FSRC, regular practice, and income all have a positive influence on the altruistic tendency. (2) Regardless of the density of regular practice and the amount of income, the FSRC increases the altruistic tendency. (3) Those who use the FSRC more often not only have higher altruistic tendencies, but their altruistic tendencies are less affected by income. (4) In the FSRC, the Four Fields for Cultivating Peace have the most significant positive influence on the altruistic tendency. (5) The above findings are still established after controlling for the influence of demographic variables such as age, gender, education level, and residence.