In the restless generation, spiritual cultivation has become an increasingly important issue. The researcher believe that the Altar-Sutra of Sixth Patriarch is a manual for spiritual cultivation. The core content of the Altar-Sutra is the Original Nature, which is how the Sixth Patriarch pointed to self-awareness and awakening. Regarding the Original Nature and Spirituality, the scholars have suggested that Spirituality is Original Nature, which is Buddha- nature. Their names are different, but they have similar meanings. This research method used Document Analysis to analyze the spiritual meaning and spiritual cultivation methods in the Altar-Sutra. From the literature review, we found that there are many definitions of spirituality. This research focuses on Life education. Therefore, it will have a dialogue and discussion with the spiritual content from the Core competence of Life education of 12-year Basic Education and the Altar-Sutra. And proffer the final conclusion. The conclusions of this study have the three points: 1. The spiritual significance of Life education of 12-year Basic Education is the spiritual characteristics of human towards eternity, the pursuit of truth and self-transcendence. The spiritual meanings of the altar corresponding to these spiritual characteristics are "the Supreme Nirvana", "apprehend one’s own Indigenous Mind" and "witnessed Original Nature to attain Buddhahood". These spiritual characteristics are somewhat the same at different levels, and some are different. 2. The spiritual cultivation of Life education of 12-year Basic Education includes spiritual consciousness and development, personality cultivation and personality integration. The "Self- deliverance by the Original Nature" of the altar corresponds to the spiritual consciousness and development. The "Self-Refuge" and "cognizance of one’s own Original Nature" of the altar implies personality cultivation and personality integration. 3. Regarding spiritual education, the Researcher of this study believe that the focus should be on cultivating " the ability to perceive the essence of things" and " the ability to take care of your own spirituality". The Researcher of this study has proposed that the "Undichotomized Dharma" and "On Stasis and Wisdom" of the altar can cultivate the ability to perceive the essence of things. "Non-deliberation, Non-appearance, Non-inhabitation" of the altar are the spiritual cultivation methods. Use these methods to take care of your own spirituality. In addition to the three spiritual characteristics of life education, spiritual meaning also has other characteristics. Since this research focuses on the spiritual cultivation of life education, it is necessary to conduct corresponding research. It is suggested that follow-up research can explore more spiritual connotations in the altar.